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donkey anti-mouse igg (h+l) secondary antibody, alexa fluor plus 555  (Thermo Fisher)


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    Structured Review

    Thermo Fisher donkey anti-mouse igg (h+l) secondary antibody, alexa fluor plus 555
    Donkey Anti Mouse Igg (H+L) Secondary Antibody, Alexa Fluor Plus 555, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/alexa+fluor+555+secondary+antibody/alexa+fluor+488/pmc12272898-14-6-13
    Average 90 stars, based on 1 article reviews
    donkey anti-mouse igg (h+l) secondary antibody, alexa fluor plus 555 - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    Incubation:

    Article Title: Evolutionary insights into the emergence of virulent Leptospira spirochetes
    Article Snippet: Cells were incubated with anti-NF-kB antibody (Thermo Fisher) overnight at 4°C. .. Cells were washed and incubated with Alexa Fluor 555 secondary antibody (Thermo Fisher) for 2 hr. .. Nuclei were stained with DAPI (1 μg/ml) for 10 min. After labeling, coverslips were set in Fluoromount G medium (Thermo Fisher).

    Article Title: Profiling the neuroimmune cascade in 3xTg-AD mice exposed to successive mild traumatic brain injuries.
    Article Snippet: Samples were incubated at 4 °C overnight with primary antibodies diluted in blocking buffer: GFAP (1:100, Diagnostic Biosystems Mob064), NeuN (1:200, Abcam ab104224), IL-1α (1:50, Thermo Fisher PA5-89037), IL-1β (1:500, Abcam ab283818), IL-13 (1:100, Abcam ab106732), KC (1:50, Thermo Fisher PA5-86508), phospho-Atf2 T71 (1:50, Thermo Fisher PA5-97332), phospho-Mek1/2 S217/S221 (1:50, Thermo Fisher PA5-105777), or phospho-tau S262/T263 (1:100, Abcam ab92627), as appropriate. .. Slides were rinsed again in TBST and incubated with either Alexa Fluor 555 and/or Alexa Fluor 488 secondary antibodies (Thermo Fisher) diluted 1:200 in blocking buffer. .. Slides were counterstained with 1 μg/mL DAPI, rinsed in water, and mounted with VECTASHIELD Antifade Mounting Medium (Vector Laboratories, Burlingame, CA).

    Article Title: Evolutionary insights into the emergence of virulent Leptospira spirochetes.
    Article Snippet: Bacteria were incubated with anti-C5b9 (Thermo Fisher) or with anti-C3b (Thermo Fisher) for 2 hr at RT. .. Bacteria were washed three times with PBS and incubated with Alexa Fluor 555 secondary antibody (Thermo Fisher) for 2 hr at RT. .. Fluorescence was analyzed using a CytoFLEX Flow Cytometer (Beckman Coulter).

    Article Title: Beta-amyloid interacts with and activates the long-form phosphodiesterase PDE4D5 in neuronal cells to reduce cAMP availability.
    Article Snippet: Cells were then treated with 5 lM FAM-Ab (Anaspec, Kidderminster, UK) for 1 or 24 h and fixed in 4% paraformaldehyde. .. Blocking with 1% BSA and 0.1% Tween80 was performed, followed by overnight incubation with a primary PDE4D antibody (Abcam, ab14613) and a 1 h incubation with an Alexa fluor 555 secondary antibody (Invitrogen, A31572). .. Counterstaining was done using DAPI dye and coverslips were mounted using fluoromount (Invitrogen).

    Article Title: Oxytocin induces embryonic diapause.
    Article Snippet: .. The next day, blastocysts were washed three times for 10 min with blocking solution and incubated with Alexa Fluor 555 (2 μg/ml; Thermo Fisher Scientific) secondary antibody for 30 min in blocking solution. ..

    Article Title: Epithelial cells maintain memory of prior infection with Streptococcus pneumoniae through di-methylation of histone H3
    Article Snippet: Immunostaining was performed overnight at +4 °C with primary antibodies (H3K4me2 1:1000; Abcam ab32356) (H3K4me2 1:1000; EpiGentek A4032) (H3K4me1 1:5000; Abcam ab176877) (H3K4me3 1:200; Diagenode C15410003) (LAMP1 1:100; Abcam ab25630) diluted in 5% BSA 0.5% Tween20, then washed three times in 0.5% Tween20 and three times in PBS. .. Cells were incubated for 1 h at room temperature with Alexa Fluor 488 secondary antibody (1:1000; Invitrogen TM A11034 or A11029) and Alexa Fluor 555 secondary antibody (1:1000; Invitrogen TM A21424) in 5% BSA 0.5% Tween20, then washed three times in 0.5% Tween20 and three times in PBS. .. Cells were incubated for 5 min at room temperature with 300 nM 4,6-diamidino-2-phenylindole (DAPI) (Invitrogen TM , D1306) diluted in PBS, then washed three times in 0.5% Tween20 and three times in PBS.

    Article Title: Evolutionary insights into the emergence of virulent Leptospira spirochetes
    Article Snippet: Bacteria were incubated with anti-C5b9 (Thermo Fisher) or with anti-C3b (Thermo Fisher) for 2 hr at RT. .. Bacteria were washed three times with PBS and incubated with Alexa Fluor 555 secondary antibody (Thermo Fisher) for 2 hr at RT. .. Fluorescence was analyzed using a CytoFLEX Flow Cytometer (Beckman Coulter).

    Article Title: Evolutionary insights into the emergence of virulent Leptospira spirochetes.
    Article Snippet: Cells were incubated with anti-NF-kB antibody (Thermo Fisher) overnight at 4 ̊C. .. Cells were washed and incubated with Alexa Fluor 555 secondary antibody (Thermo Fisher) for 2 hr. .. Nuclei were PLOS Pathogens | https://doi.org/10.1371/journal.ppat.1012161 July 17, 2024 14 / 21 stained with DAPI (1 μg/ml) for 10 min. After labeling, coverslips were set in Fluoromount G medium (Thermo Fisher).

    Blocking Assay:

    Article Title: Profiling the neuroimmune cascade in 3xTg-AD mice exposed to successive mild traumatic brain injuries.
    Article Snippet: Samples were incubated at 4 °C overnight with primary antibodies diluted in blocking buffer: GFAP (1:100, Diagnostic Biosystems Mob064), NeuN (1:200, Abcam ab104224), IL-1α (1:50, Thermo Fisher PA5-89037), IL-1β (1:500, Abcam ab283818), IL-13 (1:100, Abcam ab106732), KC (1:50, Thermo Fisher PA5-86508), phospho-Atf2 T71 (1:50, Thermo Fisher PA5-97332), phospho-Mek1/2 S217/S221 (1:50, Thermo Fisher PA5-105777), or phospho-tau S262/T263 (1:100, Abcam ab92627), as appropriate. .. Slides were rinsed again in TBST and incubated with either Alexa Fluor 555 and/or Alexa Fluor 488 secondary antibodies (Thermo Fisher) diluted 1:200 in blocking buffer. .. Slides were counterstained with 1 μg/mL DAPI, rinsed in water, and mounted with VECTASHIELD Antifade Mounting Medium (Vector Laboratories, Burlingame, CA).

    Article Title: Beta-amyloid interacts with and activates the long-form phosphodiesterase PDE4D5 in neuronal cells to reduce cAMP availability.
    Article Snippet: Cells were then treated with 5 lM FAM-Ab (Anaspec, Kidderminster, UK) for 1 or 24 h and fixed in 4% paraformaldehyde. .. Blocking with 1% BSA and 0.1% Tween80 was performed, followed by overnight incubation with a primary PDE4D antibody (Abcam, ab14613) and a 1 h incubation with an Alexa fluor 555 secondary antibody (Invitrogen, A31572). .. Counterstaining was done using DAPI dye and coverslips were mounted using fluoromount (Invitrogen).

    Article Title: Oxytocin induces embryonic diapause.
    Article Snippet: .. The next day, blastocysts were washed three times for 10 min with blocking solution and incubated with Alexa Fluor 555 (2 μg/ml; Thermo Fisher Scientific) secondary antibody for 30 min in blocking solution. ..

    Bacteria:

    Article Title: Evolutionary insights into the emergence of virulent Leptospira spirochetes.
    Article Snippet: Bacteria were incubated with anti-C5b9 (Thermo Fisher) or with anti-C3b (Thermo Fisher) for 2 hr at RT. .. Bacteria were washed three times with PBS and incubated with Alexa Fluor 555 secondary antibody (Thermo Fisher) for 2 hr at RT. .. Fluorescence was analyzed using a CytoFLEX Flow Cytometer (Beckman Coulter).

    Article Title: Evolutionary insights into the emergence of virulent Leptospira spirochetes
    Article Snippet: Bacteria were incubated with anti-C5b9 (Thermo Fisher) or with anti-C3b (Thermo Fisher) for 2 hr at RT. .. Bacteria were washed three times with PBS and incubated with Alexa Fluor 555 secondary antibody (Thermo Fisher) for 2 hr at RT. .. Fluorescence was analyzed using a CytoFLEX Flow Cytometer (Beckman Coulter).



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